{"id":35534,"date":"2017-08-09T15:01:34","date_gmt":"2017-08-09T15:01:34","guid":{"rendered":"https:\/\/virtuallearn.wpengine.com\/fhs\/?page_id=35534"},"modified":"2024-10-25T19:52:06","modified_gmt":"2024-10-25T19:52:06","slug":"section-2-aquatic-animal-health-inspections","status":"publish","type":"page","link":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/fish-health-section-blue-book-2020\/section-2-aquatic-animal-health-inspections\/","title":{"rendered":"Section 2 – Aquatic Animal Health Inspections"},"content":{"rendered":"

Chapter 1 Introduction<\/h2>\n

1.1 Introduction<\/a><\/p>\n

____________________________________________________________________________<\/p>\n

Chapter 2 Sampling<\/h2>\n

2.1 Sampling Introduction<\/a>
\n2.2 Sampling<\/a>
\n2.3 Reagents, Media, and Media Preparation<\/a>
\n2.4 Glossary<\/a>
\n2.5 References<\/a><\/p>\n

____________________________________________________________________________<\/p>\n

Chapter 3 Bacteriology<\/h2>\n

3.1 Bacteriology Introduction<\/a>
\n3.2 Aeromonas salmonicida<\/em> (Furunculosis)<\/a> Updated 2016<\/span>
\n3.3 Yersinia ruckeri<\/em> (Enteric Redmouth Disease, ERM)<\/a>
\n3.4 Edwardsiella ictaluri<\/em> (Enteric Septicemia of Catfish, ESC)<\/a>
\n3.5 Renibacterium salmoninarum<\/em> (Bacterial Kidney Disease, BKD)<\/a> Updated 2020<\/span>
\n3.6 Piscirickettsia salmonis<\/em><\/a>
\n3.7 Reagents, Media, and Media Preparation<\/a>
\n3.8 Bacterial Identification Techniques<\/a>
\n3.9 Glossary<\/a>
\n3.10 References<\/a><\/p>\n

3.A1 Laboratory Reference Flow Chart Appendix 1<\/a>
\n3.A2 Profiles Obtained with API-20E for Known Fish Pathogens<\/a>
\n3.A3.A Worksheet A \u2013 PCR Sample Data\/Log Sheet<\/a>
\n3.A3.B Worksheet B \u2013 Initial Amplification of Nucleic Acid by PCR for the Confirmation of R. salmoninarum<\/em><\/a>
\n3.A3.C Worksheet C \u2013 Nested Amplification of Nucleic Acid by PCR for the Confirmation of R. salmoninarum<\/em><\/a>
\n3.A3.D Worksheet D \u2013 Initial Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis<\/em><\/a>
\n3.A3.E Worksheet E \u2013 Nested Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis<\/em><\/a>
\n3.A3.F Worksheet F \u2013 Direct Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis<\/em><\/a>
\n3.A3.G Worksheet G \u2013 Photodocumentation of the PCR Product Gel<\/a><\/p>\n

____________________________________________________________________________<\/p>\n

Chapter 4 Virology<\/h2>\n

4.1 Virology Introduction<\/a>
\n4.2 Selection of Appropriate Cell Lines<\/a>
\n4.3 Cell Culture<\/a>
\n4.4 Sample Processing Procedures<\/a>
\n4.5 Screening Method for Viral Isolation<\/a>
\n4.6 Identification of Viruses<\/a>
\n4.7 Serum Neutralizations<\/a>
\n4.8 Indirect Fluorescent Antibody (IFAT) Procedure<\/a>
\n4.9 Reagents and Media<\/a>
\n4.10 Glossary<\/a>
\n4.11 References<\/a><\/p>\n

4.A1.A Worksheet A \u2013 PCR Sample Data\/Log Sheet<\/a>
\n4.A1.B Worksheet B \u2013 Amplification of Nucleic Acid by PCR for the Confirmation of Viral Fish Pathogens<\/a>
\n4.A1.B.1 Worksheet B.1 \u2013 Infectious Hematopoietic Necrosis Virus (IHNV)<\/a>
\n4.A1.B.2 Worksheet B.2 \u2013 Infectious Pancreatic Necrosis Virus (IPNV)<\/a>
\n4.A1.B.3 Worksheet B.3 \u2013 Infectious Salmon Anemia Virus (ISAV)<\/a>
\n4.A1.B.4 Worksheet B.4 \u2013 Largemouth Bass Virus (LMBV)<\/a>
\n4.A1.B.5 Worksheet B.5 \u2013 Oncorhynchus masou<\/em> Virus (OMV)<\/a>
\n4.A1.B.6 Worksheet B.6 \u2013 Spring Viremia of Carp Virus (SVCV)<\/a>
\n4.A1.B.7 Worksheet B.7 \u2013 Viral Hemorrhagic Septicemia Virus (VHSV) RT-PCR<\/a>
\n4.A1.B.8 SOPs for Viral Hemorrhagic Septicemia Virus (VHSV) Real-time RT-PCR<\/a> Updated 2020<\/span>
\n4.A1.C Worksheet C \u2013 Photodocumentation of the PCR Product Gel<\/a>
\n<\/a>4.A2 Cell Enumeration<\/a><\/p>\n

____________________________________________________________________________<\/p>\n

Chapter 5 Parasitology<\/h2>\n

5.1 Parasitology Introduction<\/a>
\n5.2 Myxobolus cerebralis<\/em> (Whirling Disease)<\/a>\u00a0Updated 2014<\/span>
\n5.3 Ceratonova shasta<\/em> (Ceratomyxosis)<\/a>
\n5.4 Tetracapsuloides\u00a0bryosalmonae<\/em> (Proliferative Kidney Disease)<\/a>
\n5.5 Bothriocephalus acheilognathi<\/em> (Bothriocephaloisis, Asian Tapeworm)<\/a>
\n5.6\u00a0 Media and Reagents<\/a>
\n5.7 Glossary<\/a>
\n5.8 References<\/a><\/p>\n

____________________________________________________________________________<\/p>\n

Chapter 6 Polymerase Chain Reaction<\/h2>\n

6.1 Polymerase Chain Reaction (PCR) Introduction<\/a>
\n6.2 PCR <\/a>\u2013 <\/a>Quality Assurance\/Quality Control<\/a>
\n6.3 PCR Protocols<\/a>
\n6.4 Reagents<\/a>
\n6.5 Analysis of Extracted DNA Using an UV Spectrophotometer<\/a>
\n6.6 Table of PCR Protocols<\/a>
\n6.7 References<\/a><\/p>\n

____________________________________________________________________________<\/p>\n

Section 2 Appendices<\/h2>\n

APPENDIX 1 THE COMMITTEE<\/h3>\n

A1.1 Format of the Handbook<\/a>
\nA1.2 Handbook Revision and Oversight Committee<\/a>
\nA1.3 Process for Amending the Handbook<\/a><\/p>\n

APPENDIX 2 HANDBOOK COMMITTEES<\/h3>\n

A2.1 2000 \u2013 2002 Handbook Development Committee<\/a>
\nA2.2 2002 \u2013 2003 Handbook Development Committee<\/a>
\nA2.3 2003 \u2013 2004 Handbook Development Committee<\/a>
\nA2.4 2004 \u2013 2005 Handbook Development Committee<\/a>
\nA2.5 2006 \u2013 2007 Handbook Development Committee<\/a>
\nA2.6 2008 \u2013 2010 Handbook Development Committee<\/a>
\nA2.7 2011 \u2013 2012 Handbook Development Committee<\/a>
\nA2.8 2013 \u2013 2014 Handbook Development Committee<\/a>
\nA2.9 2019 \u2013 2020 Handbook Revision and Oversight Committee<\/a>\u00a0Updated 2020<\/span><\/p>\n

APPENDIX 3 POSITION STATEMENTS<\/h3>\n

A3.1 Position Statements Introduction<\/a>
\nA3.2 Sampling Position Statements<\/a>
\nA3.3 Bacteriology Position Statements<\/a> Updated 2020<\/span>
\nA3.4 Virology Position Statements<\/a> Updated 2020<\/span>
\nA 3.5 Parasitology Position Statements<\/a>\u00a0Updated 2020<\/span>
\nA3.6 Polymerase Chain Reaction Position Statement<\/a> Updated 2020<\/span>
\nA3.7 The Handbook and Oversight Committee Position Statements<\/a>\u00a0Updated 2020<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Chapter 1 Introduction 1.1 Introduction ____________________________________________________________________________ Chapter 2 Sampling 2.1 Sampling Introduction 2.2 Sampling 2.3 Reagents, Media, and Media Preparation 2.4 Glossary 2.5 References ____________________________________________________________________________ Chapter 3 Bacteriology 3.1 Bacteriology Introduction 3.2 Aeromonas salmonicida (Furunculosis) Updated 2016 3.3 Yersinia ruckeri (Enteric Redmouth Disease, ERM) 3.4 Edwardsiella ictaluri (Enteric Septicemia of Catfish, ESC) 3.5 Renibacterium salmoninarum […]<\/p>\n","protected":false},"author":62,"featured_media":0,"parent":35345,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"class_list":["post-35534","page","type-page","status-publish","hentry","odd","first-child","last-child"],"acf":[],"_links":{"self":[{"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/pages\/35534","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/users\/62"}],"replies":[{"embeddable":true,"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/comments?post=35534"}],"version-history":[{"count":0,"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/pages\/35534\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/pages\/35345"}],"wp:attachment":[{"href":"https:\/\/oldunitsstaging.fisheries.org\/fhs\/wp-json\/wp\/v2\/media?parent=35534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}